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How to use a spectrometer to measure substances
Most spectrophotometers require you to place a sample in a cuvette—a small, clear container that holds your liquid. Then, you'll select a wavelength of light to send through the sample. Spectrophotometry is an experimental technique that is used to measure the concentration of solutes in a specific solution by calculating the amount of light absorbed by those solutes. This technique is powerful because certain compounds will absorb different wavelengths of light at different. A spectrometer is an analytical tool used across various scientific disciplines to measure how a substance interacts with light. This guide makes spectroscopy simple by showing you how to use teaching tools and real experiments. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength.